Flow Cytometry and Cell Sorting
This service supports researchers in the design of assays, sample analysis, and presentation of results in the field of flow cytometry. Among its main functions, it is in charge of developing flow cytometry protocols adapted to specific problems based on the nature and dynamics of the sample.

The service is in charge of carrying out the maintenance of the cytometers and separators of the service, managing the reservations for the equipment, advising on the analysis of samples and the publication of the results, and identifying technical advances in the field of flow cytometry. Among the studies that can be carried out, the identification of cell populations by marking intra- or extracellular antigens, cell viability analysis, apoptosis studies (Annexin-V/Propidium Iodide, TUNEL, determination of the mitochondrial membrane potential, detection of sub-G0 peak, etc.), oxidative stress studies using fluorescent probes, cell cycle study, determination of the cell proliferative index by labelling with BrdU, EdU, etc., gene expression studies guided by fluorescent proteins (GFP, YFP), intracellular signalling studies, quantification of cytokines and other soluble proteins, intracellular Ca2+ mobilization studies, and sorting assays in all identified cell and subcellular populations.


CONTACT
Cajal Institute Headquarters: Avda Doctor Arce 37, 28002, Madrid
Ci2A building : Avda. de León 1, 28805, Alcalá de Henares, Madrid
direccion.ic@csic.es
direccion.ci2a@csic.es
Phone: +34 91 585 47 52 (Cajal Institute headquarters)